Coding
Part:BBa_K4165051:Design
Designed by: Esraa Elmligy Group: iGEM22_CU_Egypt (2022-09-30)
HtrA1 Switch 31
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 589
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 572
Illegal AgeI site found at 308 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was optimized to be suitable for expression on E.coli.
Source
Synthetic fusion protein